1AL9

NMR STUDY OF DNA (5'-D(*AP*CP*GP*TP*AP*CP*GP*T)-3') SELF-COMPLEMENTARY DUPLEX COMPLEXED WITH A BIS-DAUNORUBICIN, MINIMIZED AVERAGE STRUCTURE

Summary for 1AL9

• Entry DOI: 10.2210/pdb1al9/pdb
• Related: 1AMD
• Descriptor: DNA (5'-D(*AP*CP*GP*TP*AP*CP*GP*T)-3'), 4-METHYLBENZYL-N-BIS[DAUNOMYCIN] (2 entities in total)
• Functional Keywords: complex (deoxyribonucleic acid-drug), bis-intercalator, daunorubicin, dna, deoxyribonucleic acid
• Total number of polymer chains: 2
• Total formula weight: 6012.42

Authors

Robinson, H.,Wang, A.H.-J. (deposition date: 1997-06-12, release date: 1997-09-17, Last modification date: 2024-05-22)

Primary citation

Robinson, H.,Priebe, W.,Chaires, J.B.,Wang, A.H.
Binding of two novel bisdaunorubicins to DNA studied by NMR spectroscopy.
Biochemistry, 36:8663-8670, 1997

PubMed Abstract: In the search for new generations of anthracycline drugs, lower cytotoxic side effects and higher activity against resistant cancer cells are two major goals. A new class of bis-intercalating anthracycline drugs has been designed, synthesized, and shown to have promising activity against multidrug-resistant cells. Two daunorubicins symmetrically linked together via a p-xylenyl group, either at their N3' (compound WP631) or N4' sites (compound WP652), exhibit extraordinary DNA binding affinities. We have used high-resolution NRM studies to understand the DNA binding mode of these two new bis-daunorubicin anticancer compounds. The structures of the WP631-d(ACGTACGT)2 and the WP652-d(TGTACA)2 complexes have been determined by NOE-restrained refinement. WP631 binds strongly to the 5'-CG(A/T)(A/T)CG hexanucleotide sequence, with the aglycons intercalated between the two CpG sites at both ends of the hexanucleotide sequence. The overall conformation of the WP631-d(CGTACG)2 part is remarkably similar to the crystal structure of the 2:1 complex of daunorubicin and d(CGTACG)2, as predicted previously [Gao, Y.-G., & Wang, A.H.H. (1996), J. Biomol. Struct. Dyn. 13, 103-117]. In contrast, the related bis-intercalator WP652 prefers the 5'-PyGTPu tetranucleotide sequence, with the aglycons intercalated between the PypG and TpPu sites. The binding of WP652 to DNA results in a severely distroted B-DNA duplex with the p-xylenyl tether moiety significantly protruded away from the bottom of the minor groove. While WP652 in some ways behaves similarly to other anticancer bis-intercalating antibiotics (e.g., triostine A and echinomycin), the detailed interactions between those two classes of bis-intercalators are quite different.

PubMed: 9289011
DOI: 10.1021/bi970842j

Experimental method

SOLUTION NMR