1AJD

THREE-DIMENSIONAL STRUCTURE OF THE D153G MUTANT OF E. COLI ALKALINE PHOSPHATASE: A MUTANT WITH WEAKER MAGNESIUM BINDING AND INCREASED CATALYTIC ACTIVITY

Summary for 1AJD

DescriptorALKALINE PHOSPHATASE INTERMEDIATE II OF HOLO ENZYME, ZINC ION, MAGNESIUM ION, ... (4 entities in total)
Functional Keywordsnon specific mono-esterase
Biological sourceEscherichia coli
Cellular locationPeriplasm P00634
Total number of polymer chains2
Total molecular weight94384.94
Authors
Dealwis, C.G.,Chen, L.,Abad-Zapatero, C. (deposition date: 1995-08-18, release date: 1995-11-14, Last modification date: 2011-07-13)
Primary citation
Dealwis, C.G.,Brennan, C.,Christianson, K.,Mandecki, W.,Abad-Zapatero, C.
Crystallographic analysis of reversible metal binding observed in a mutant (Asp153-->Gly) of Escherichia coli alkaline phosphatase.
Biochemistry, 34:13967-13973, 1995
PubMed: 7577993 (PDB entries with the same primary citation)
DOI: 10.1021/bi00043a001
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.5 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliers171.0%8.4%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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