1AB8

RAT TYPE II ADENYLYL CYCLASE C2 DOMAIN/FORSKOLIN COMPLEX

1AB8 の概要

• エントリーDOI: 10.2210/pdb1ab8/pdb
• 分子名称: ADENYLYL CYCLASE, FORSKOLIN (3 entities in total)
• 機能のキーワード: lyase, adenylyl cyclase, plasmid, complex (transferase-inhibitor)
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Membrane ; Multi-pass membrane protein : P26769
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49716.56

構造登録者

Zhang, G.,Hurley, J.H. (登録日: 1997-02-04, 公開日: 1997-05-15, 最終更新日: 2024-02-07)

主引用文献

Zhang, G.,Liu, Y.,Ruoho, A.E.,Hurley, J.H.
Structure of the adenylyl cyclase catalytic core.
Nature, 386:247-253, 1997

PubMed Abstract: Mammalian adenylyl cyclases contain two conserved regions, C1 and C2, which are responsible for forskolin- and G-protein-stimulated catalysis. The structure of the C2 catalytic region of type II rat adenylyl cyclase has an alpha/beta class fold in a wreath-like dimer, which has a central cleft. Two forskolin molecules bind in hydrophobic pockets at the ends of cleft. The central part of the cleft is lined by charged residues implicated in ATP binding. Forskolin appears to activate adenylyl cyclase by promoting the assembly of the active dimer and by direct interaction within the catalytic cleft. Other adenylyl cyclase regulators act at the dimer interface or on a flexible C-terminal region.

PubMed: 9069282
DOI: 10.1038/386247a0

実験手法

X-RAY DIFFRACTION (2.2 Å)