1A9L

SOLUTION STRUCTURE OF A SUBSTRATE FOR THE ARCHAEAL PRE-TRNA SPLICING ENDONUCLEASES: THE BULGE-HELIX-BULGE MOTIF, NMR, 12 STRUCTURES

Summary for 1A9L

• Entry DOI: 10.2210/pdb1a9l/pdb
• Descriptor: PRE-TRNA BULGE-HELIX-BULGE MOTIF (1 entity in total)
• Functional Keywords: archaeal bulge-helix-bulge motif, pre-trna splicing, archaea, rna structure, ribonucleic acid, rna
• Total number of polymer chains: 1
• Total formula weight: 12317.42

Authors

Diener, J.L.,Moore, P.B. (deposition date: 1998-04-07, release date: 1998-06-17, Last modification date: 2024-05-22)

Primary citation

Diener, J.L.,Moore, P.B.
Solution structure of a substrate for the archaeal pre-tRNA splicing endonucleases: the bulge-helix-bulge motif.
Mol.Cell, 1:883-894, 1998

PubMed Abstract: The structure of the bulge-helix-bulge motif that constitutes the intron/exon splice site in H. volcanii pre-tRNATrp has been determined by NMR spectroscopy. The conformations of the two 3 nt bulges, where the pre-tRNA is cleaved, are stabilized by stacking interactions between bulge nucleotides and bases in the adjacent Watson-Crick helices and by a network of backbone hydrogen bonds. Both bulges are presented on the same minor groove face of the central 4 bp helix, and the overall structure has approximate two-fold symmetry, which makes it well-suited for attack by archaeal splicing endonucleases, which are symmetric dimers.

PubMed: 9660971
DOI: 10.1016/S1097-2765(00)80087-8

Experimental method

SOLUTION NMR