1A76

FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII

1A76 の概要

• エントリーDOI: 10.2210/pdb1a76/pdb
• 分子名称: FLAP ENDONUCLEASE-1 PROTEIN, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: 5'-3' exo/endo nuclease, dna replication, rth, rad27, dna repair, 5'-3' exo-endo nuclease complex
• 由来する生物種: Methanocaldococcus jannaschii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37695.35

構造登録者

Hwang, K.Y.,Baek, K.,Kim, H.,Cho, Y. (登録日: 1998-03-20, 公開日: 1999-08-03, 最終更新日: 2024-02-07)

主引用文献

Hwang, K.Y.,Baek, K.,Kim, H.Y.,Cho, Y.
The crystal structure of flap endonuclease-1 from Methanococcus jannaschii.
Nat.Struct.Biol., 5:707-713, 1998

PubMed Abstract: Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 A resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA.

PubMed: 9699635
DOI: 10.1038/1406

実験手法

X-RAY DIFFRACTION (2 Å)