1A54

PHOSPHATE-BINDING PROTEIN MUTANT A197C LABELLED WITH A COUMARIN FLUOROPHORE AND BOUND TO DIHYDROGENPHOSPHATE ION

1A54 の概要

• エントリーDOI: 10.2210/pdb1a54/pdb
• 分子名称: Phosphate-binding protein PstS, DIHYDROGENPHOSPHATE ION, N-[2-(1-MALEIMIDYL)ETHYL]-7-DIETHYLAMINOCOUMARIN-3-CARBOXAMIDE, ... (4 entities in total)
• 機能のキーワード: phosphotransferase, transport, coumarin, fluorophore
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34970.05

構造登録者

Hirshberg, M.,Henrick, K.,Lloyd-Haire, L.,Vasisht, N.,Brune, M.,Corrie, J.E.T.,Webb, M.R. (登録日: 1998-02-19, 公開日: 1998-10-14, 最終更新日: 2024-12-25)

主引用文献

Hirshberg, M.,Henrick, K.,Haire, L.L.,Vasisht, N.,Brune, M.,Corrie, J.E.,Webb, M.R.
Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate.
Biochemistry, 37:10381-10385, 1998

PubMed Abstract: Crystal structures are presented for the A197C mutant of Escherichia coli phosphate binding protein (PBP) and the same mutant labeled at Cys197 with N-[2-(1-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Both proteins are complexed with inorganic phosphate. The latter molecule, MDCC-PBP, exhibits a large increase in fluorescence on binding inorganic phosphate. The resulting high-fluorescence state of the coumarin and the ability of this coumarin to monitor the conformational changes associated with inorganic phosphate binding are interpreted in terms of the specific interactions of MDCC with the protein. The structure helps to explain why this particular label gives a high-fluorescence state on binding inorganic phosphate, while several other related labels do not, and hence aids our general understanding of environmentally sensitive fluorescence probes on proteins.

PubMed: 9671506
DOI: 10.1021/bi980428z

実験手法

X-RAY DIFFRACTION (1.6 Å)