1A40

PHOSPHATE-BINDING PROTEIN WITH ALA 197 REPLACED WITH TRP

Summary for 1A40

DescriptorPHOSPHATE-BINDING PERIPLASMIC PROTEIN PRECURSOR, PHOSPHATE ION (3 entities in total)
Functional Keywordsphosphate-binding protein, mutagenesis, charge complementary, kinetics
Biological sourceEscherichia coli
Total number of polymer chains1
Total molecular weight34667.7
Authors
Ledivina, P.S.,Wang, Z.,Tsai, A.,Koehl, E.,Quiocho, F.A. (deposition date: 1998-02-10, release date: 1999-03-23, Last modification date: 2011-07-13)
Primary citation
Ledvina, P.S.,Tsai, A.L.,Wang, Z.,Koehl, E.,Quiocho, F.A.
Dominant role of local dipolar interactions in phosphate binding to a receptor cleft with an electronegative charge surface: equilibrium, kinetic, and crystallographic studies.
Protein Sci., 7:2550-2559, 1998
PubMed: 9865949 (PDB entries with the same primary citation)
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.7 Å)
?

Structure validation

ClashscoreRamachandran outliersSidechain outliersRSRZ outliers605.5%2.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 1a40
no rotation
Molmil generated image of 1a40
rotated about x axis by 90°
Molmil generated image of 1a40
rotated about y axis by 90°