1A3O

ARTIFICIAL MUTANT (ALPHA Y42H) OF DEOXY HEMOGLOBIN

Summary for 1A3O

• Entry DOI: 10.2210/pdb1a3o/pdb
• Descriptor: HEMOGLOBIN (ALPHA CHAIN), HEMOGLOBIN (BETA CHAIN), PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• Functional Keywords: oxygen transport, heme, respiratory protein, erythrocyte
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 4
• Total formula weight: 64497.00

Authors

Tame, J.,Vallone, B. (deposition date: 1998-01-22, release date: 1998-04-29, Last modification date: 2024-02-07)

Primary citation

Tame, J.R.,Vallone, B.
The structures of deoxy human haemoglobin and the mutant Hb Tyralpha42His at 120 K.
Acta Crystallogr.,Sect.D, 56:805-811, 2000

PubMed Abstract: The structures of deoxy human haemoglobin and an artificial mutant (Tyralpha42-->His) have been solved at 120 K. While overall agreement between these structures and others in the PDB is very good, certain side chains are found to be shifted, absent from the electron-density map or in different rotamers. Non-crystallographic symmetry (NCS) is very well obeyed in the native protein, but not around the site of the changed residue in the mutant. NCS is also not obeyed by the water molecule invariably found in the alpha-chain haem pocket in room-temperature crystal structures of haemoglobin. At 120 K, this water molecule disappears from one alpha chain in the asymmetric unit but not the other.

PubMed: 10930827
DOI: 10.1107/S0907444900006387

Experimental method

X-RAY DIFFRACTION (1.8 Å)