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1A1T

STRUCTURE OF THE HIV-1 NUCLEOCAPSID PROTEIN BOUND TO THE SL3 PSI-RNA RECOGNITION ELEMENT, NMR, 25 STRUCTURES

Summary for 1A1T
Entry DOI10.2210/pdb1a1t/pdb
DescriptorSL3 STEM-LOOP RNA, NUCLEOCAPSID PROTEIN, ZINC ION (3 entities in total)
Functional Keywordsnucleocapsid protein, complex (nucleocapsid protein-rna), stem-loop rna, viral protein-rna complex, viral protein/rna
Biological sourceHuman immunodeficiency virus 1
Total number of polymer chains2
Total formula weight12995.23
Authors
De Guzman, R.N.,Wu, Z.R.,Stalling, C.C.,Pappalardo, L.,Borer, P.N.,Summers, M.F. (deposition date: 1997-12-15, release date: 1998-06-17, Last modification date: 2024-05-22)
Primary citationDe Guzman, R.N.,Wu, Z.R.,Stalling, C.C.,Pappalardo, L.,Borer, P.N.,Summers, M.F.
Structure of the HIV-1 nucleocapsid protein bound to the SL3 psi-RNA recognition element.
Science, 279:384-388, 1998
Cited by
PubMed Abstract: The three-dimensional structure of the human immunodeficiency virus-type 1 (HIV-1) nucleocapsid protein (NC) bound to the SL3 stem-loop recognition element of the genomic Psi RNA packaging signal has been determined by heteronuclear magnetic resonance spectroscopy. Tight binding (dissociation constant, approximately 100 nM) is mediated by specific interactions between the amino- and carboxyl-terminal CCHC-type zinc knuckles of the NC protein and the G7 and G9 nucleotide bases, respectively, of the G6-G7-A8-G9 RNA tetraloop. A8 packs against the amino-terminal knuckle and forms a hydrogen bond with conserved Arg32, and residues Lys3 to Arg10 of NC form a 310 helix that binds to the major groove of the RNA stem and also packs against the amino-terminal zinc knuckle. The structure provides insights into the mechanism of viral genome recognition, explains extensive amino acid conservation within NC, and serves as a basis for the development of inhibitors designed to interfere with genome encapsidation.
PubMed: 9430589
DOI: 10.1126/science.279.5349.384
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

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