1A1R

HCV NS3 PROTEASE DOMAIN:NS4A PEPTIDE COMPLEX

1A1R の概要

• エントリーDOI: 10.2210/pdb1a1r/pdb
• 分子名称: NS3 PROTEIN, NS4A PROTEIN, ZINC ION, ... (4 entities in total)
• 機能のキーワード: viral protein, serine protease, nonstructural proteins, cofactor peptide, helicase
• 由来する生物種: Hepatitis C virus; 詳細
• 細胞内の位置: Core protein p21: Host endoplasmic reticulum membrane ; Single-pass membrane protein . Core protein p19: Virion . Envelope glycoprotein E1: Virion membrane ; Single-pass type I membrane protein . Envelope glycoprotein E2: Virion membrane ; Single-pass type I membrane protein . p7: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Protease NS2-3: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Serine protease NS3: Host endoplasmic reticulum membrane ; Peripheral membrane protein . Non-structural protein 4A: Host endoplasmic reticulum membrane ; Single-pass type I membrane protein . Non-structural protein 4B: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Non-structural protein 5A: Host endoplasmic reticulum membrane; Peripheral membrane protein. RNA-directed RNA polymerase: Host endoplasmic reticulum membrane ; Single-pass type I membrane protein : P27958 P27958
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 47008.85

構造登録者

Kim, J.L.,Morgenstern, K.A.,Lin, C.,Fox, T.,Dwyer, M.D.,Landro, J.A.,Chambers, S.P.,Markland, W.,Lepre, C.A.,O'Malley, E.T.,Harbeson, S.L.,Rice, C.M.,Murcko, M.A.,Caron, P.R.,Thomson, J.A. (登録日: 1997-12-15, 公開日: 1998-06-17, 最終更新日: 2024-02-07)

主引用文献

Kim, J.L.,Morgenstern, K.A.,Lin, C.,Fox, T.,Dwyer, M.D.,Landro, J.A.,Chambers, S.P.,Markland, W.,Lepre, C.A.,O'Malley, E.T.,Harbeson, S.L.,Rice, C.M.,Murcko, M.A.,Caron, P.R.,Thomson, J.A.
Crystal structure of the hepatitis C virus NS3 protease domain complexed with a synthetic NS4A cofactor peptide.
Cell(Cambridge,Mass.), 87:343-355, 1996

PubMed Abstract: An estimated 1% of the global human population is infected by hepatitis C viruses (HCVs), and there are no broadly effective treatments for the debilitating progression of chronic hepatitis C. A serine protease located within the HCV NS3 protein processes the viral polyprotein at four specific sites and is considered essential for replication. Thus, it emerges as an attractive target for drug design. We report here the 2.5 angstrom resolution X-ray crystal structure of the NS3 protease domain complexed with a synthetic NS4A activator peptide. The protease has a chymotrypsin-like fold and features a tetrahedrally coordinated metal ion distal to the active site. The NS4A peptide intercalates within a beta sheet of the enzyme core.

PubMed: 8861917
DOI: 10.1016/S0092-8674(00)81351-3

実験手法

X-RAY DIFFRACTION (2.5 Å)