1A0H

THE X-RAY CRYSTAL STRUCTURE OF PPACK-MEIZOTHROMBIN DESF1: KRINGLE/THROMBIN AND CARBOHYDRATE/KRINGLE/THROMBIN INTERACTIONS AND LOCATION OF THE LINKER CHAIN

1A0H の概要

• エントリーDOI: 10.2210/pdb1a0h/pdb
• 関連するBIRD辞書のPRD_ID: PRD_000020
• 分子名称: MEIZOTHROMBIN, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, D-phenylalanyl-N-[(2S,3S)-6-{[amino(iminio)methyl]amino}-1-chloro-2-hydroxyhexan-3-yl]-L-prolinamide, ... (5 entities in total)
• 機能のキーワード: serine protease, coagulation, thrombin, prothrombin, meizothrombin, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Bos taurus (cattle); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 97038.29

構造登録者

Martin, P.D.,Malkowski, M.G.,Box, J.,Esmon, C.T.,Edwards, B.F.P. (登録日: 1997-11-30, 公開日: 1998-06-17, 最終更新日: 2024-11-13)

主引用文献

Martin, P.D.,Malkowski, M.G.,Box, J.,Esmon, C.T.,Edwards, B.F.
New insights into the regulation of the blood clotting cascade derived from the X-ray crystal structure of bovine meizothrombin des F1 in complex with PPACK.
Structure, 5:1681-1693, 1997

PubMed Abstract: The conversion of prothrombin to thrombin by factor Xa is the penultimate step in the blood clotting cascade. In vivo, where the conversion occurs primarily on activated platelets in association with factor Va and Ca2+ ions, meizothrombin is the major intermediate of the two step reaction. Meizothrombin rapidly loses the fragment 1 domain (F1) by autolysis to become meizothrombin des F1 (mzTBN-F1). The physiological properties of mzTBN-F1 differ dramatically from those of thrombin due to the presence of prothrombin fragment 2 (F2), which remains covalently attached to the activated thrombin domain in mzTBN-F1.

PubMed: 9438869
DOI: 10.1016/S0969-2126(97)00314-6

実験手法

X-RAY DIFFRACTION (3.2 Å)