1Z4N

Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate cocrystallized with Fluoride

Summary for 1Z4N

• Entry DOI: 10.2210/pdb1z4n/pdb
• Related: 1Z4O
• Descriptor: Beta-phosphoglucomutase, MAGNESIUM ION, 1-O-phosphono-alpha-D-galactopyranose, ... (4 entities in total)
• Functional Keywords: isomerase, beta-phosphoglucomutase
• Biological source: Lactococcus lactis
• Cellular location: Cytoplasm : P71447
• Total number of polymer chains: 2
• Total formula weight: 49048.07

Authors

Tremblay, L.W.,Zhang, G.,Dai, J.,Dunaway-Mariano, D.,Allen, K.N. (deposition date: 2005-03-16, release date: 2005-04-19, Last modification date: 2023-08-23)

Primary citation

Tremblay, L.W.,Zhang, G.,Dai, J.,Dunaway-Mariano, D.,Allen, K.N.
Chemical Confirmation of a Pentavalent Phosphorane in Complex with beta-Phosphoglucomutase
J.Am.Chem.Soc., 127:5298-5299, 2005

PubMed Abstract: This communication reports the X-ray crystal structure of the alpha-d-galactose-1-phosphate complex with that of Lactococcus lactis beta-phosphoglucomutase (beta-PGM) crystallized in the presence of Mg2+ cofactor and the enzyme-to-phosphorus ratio determined by protein and phosphate analyses of the crystalline complex. The 1:1 ratio determined for this complex was compared to the 1:2 ratio determined for the crystals of beta-PGM grown in the presence of substrate and Mg2+ cofactor. This result verifies the published structure assignment of this latter complex as the phosphorane adduct formed by covalent bonding between the active site Asp8 carboxylate to the C(1)phosphorus of the beta-glucose 1,6-bisphosphate ligand and rules out the proposal of a beta-PGM-glucose-6-phosphate-1-MgF3- complex.

PubMed: 15826149
DOI: 10.1021/ja0509073

Experimental method

X-RAY DIFFRACTION (1.97 Å)