1Z4N
Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate cocrystallized with Fluoride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.143, 36.451, 105.119 |
| Unit cell angles | 90.00, 94.35, 90.00 |
Refinement procedure
| Resolution | 100.000 - 1.970 |
| R-factor | 0.236 |
| Rwork | 0.207 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1O08.pdb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.049 | 0.336 |
| Number of reflections | 31187 | |
| <I/σ(I)> | 21.3 | 5.6 |
| Completeness [%] | 95.3 | 93 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 200 mM sodium acetate, 30% PEG 4000, 10 mM magnesium dicloride, 100 mM ammonium fluoride, 50 mM alpha-galactose 1-phosphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
