1XAK
STRUCTURE OF THE SARS-CORONAVIRUS ORF7A ACCESSORY PROTEIN
Summary for 1XAK
Entry DOI | 10.2210/pdb1xak/pdb |
Descriptor | SARS ORF7A ACCESSORY PROTEIN (2 entities in total) |
Functional Keywords | i-set ig domain, beta sandwich, structural genomics, psi, protein structure initiative, midwest center for structural genomics, mcsg, viral protein |
Biological source | SARS coronavirus |
Cellular location | Virion: P59635 |
Total number of polymer chains | 1 |
Total formula weight | 9424.54 |
Authors | Nelson, C.A.,Lee, C.A.,Fremont, D.H.,Midwest Center for Structural Genomics (MCSG) (deposition date: 2004-08-26, release date: 2004-10-05, Last modification date: 2024-10-30) |
Primary citation | Nelson, C.A.,Pekosz, A.,Lee, C.A.,Diamond, M.S.,Fremont, D.H. Structure and intracellular targeting of the SARS-coronavirus Orf7a accessory protein. Structure, 13:75-85, 2005 Cited by PubMed Abstract: The open reading frame (ORF) 7a of the SARS-associated coronavirus (SARS-CoV) encodes a unique type I transmembrane protein of unknown function. We have determined the 1.8 A resolution crystal structure of the N-terminal ectodomain of orf7a, revealing a compact seven-stranded beta sandwich unexpectedly similar in fold and topology to members of the Ig superfamily. We also demonstrate that, in SARS-CoV- infected cells, the orf7a protein is expressed and retained intracellularly. Confocal microscopy studies using orf7a and orf7a/CD4 chimeras implicate the short cytoplasmic tail and transmembrane domain in trafficking of the protein within the endoplasmic reticulum and Golgi network. Taken together, our findings provide a structural and cellular framework in which to explore the role of orf7a in SARS-CoV pathogenesis. PubMed: 15642263DOI: 10.1016/j.str.2004.10.010 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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