1XAK

STRUCTURE OF THE SARS-CORONAVIRUS ORF7A ACCESSORY PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	110
Detector technology	CCD
Collection date	2003-11-09
Detector	ADSC QUANTUM 4
Wavelength(s)	0.90000
Spacegroup name	P 31
Unit cell lengths	37.100, 37.100, 55.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.550 - 1.800
R-factor	0.223
Rwork	0.223
R-free	0.27500
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.880
High resolution limit [Å]	1.800	1.800
Number of reflections	7744
<I/σ(I)>	34.9	5.2
Completeness [%]	98.7	100
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.35	293.15	16% PEG 400, 100 mM sodium acetate, pH 5.35, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K