1X7T

Structure of TTR R104H: a non-amyloidogenic variant with protective clinical effects

Summary for 1X7T

• Entry DOI: 10.2210/pdb1x7t/pdb
• Related: 1X7S
• Descriptor: Transthyretin (2 entities in total)
• Functional Keywords: transthyretin, familial amyloidotic polyneuropathy, amyloid, transport protein
• Biological source: Homo sapiens (human)
• Cellular location: Secreted: P02766
• Total number of polymer chains: 2
• Total formula weight: 27516.63

Authors

Neto-Silva, R.M.,Macedo-Ribeiro, S.,Pereira, P.J.B.,Coll, M.,Saraiva, M.J.,Damas, A.M. (deposition date: 2004-08-16, release date: 2005-03-22, Last modification date: 2023-08-23)

Primary citation

Neto-Silva, R.M.,Macedo-Ribeiro, S.,Pereira, P.J.,Coll, M.,Saraiva, M.J.,Damas, A.M.
X-ray crystallographic studies of two transthyretin variants: further insights into amyloidogenesis.
Acta Crystallogr.,Sect.D, 61:333-339, 2005

PubMed Abstract: Transthyretin (TTR) is a homotetrameric plasma protein that, as a result of a set of not yet fully characterized conformational changes, forms fibrillar aggregates that are the major protein component of amyloid deposits. More than 80 mutations associated with TTR amyloid deposition have been described in the literature. X-ray crystallography was used to elucidate the three-dimensional structure of two important TTR variants: TTR Y78F, an amyloidogenic protein, and TTR R104H, which is associated with a protective effect over the amyloidogenic V30M mutation. The structures of those two TTR variants have been determined in space group P2(1)2(1)2 to 1.55 and 1.60 angstroms resolution, respectively, using molecular-replacement techniques. Detailed analysis of the protein model for TTR Y78F indicates a destabilization of the contacts between the alpha-helix and AB loop and the body of the molecule, intimately related to the amyloidogenic nature; contrastingly, in the TTR R104H variant new contacts involving the N-terminal region and His104 are clearly antagonists of amyloid formation.

PubMed: 15735344
DOI: 10.1107/S0907444904034316

Experimental method

X-RAY DIFFRACTION (1.6 Å)