1UJZ

Crystal structure of the E7_C/Im7_C complex; a computationally designed interface between the colicin E7 DNase and the Im7 Immunity protein

Summary for 1UJZ

• Entry DOI: 10.2210/pdb1ujz/pdb
• Related: 7CEI
• Descriptor: Designed Colicin E7 immunity protein, Designed Colicin E7 DNase (3 entities in total)
• Functional Keywords: computational design, redesigned protein-protein interface, specificity, molecular recognition, protein complex, immune system
• Biological source: Escherichia coli; More
• Total number of polymer chains: 2
• Total formula weight: 24661.70

Authors

Kortemme, T.,Joachimiak, L.A.,Bullock, A.N.,Schuler, A.D.,Stoddard, B.L.,Baker, D. (deposition date: 2003-08-13, release date: 2004-04-06, Last modification date: 2023-10-25)

Primary citation

Kortemme, T.,Joachimiak, L.A.,Bullock, A.N.,Schuler, A.D.,Stoddard, B.L.,Baker, D.
Computational redesign of protein-protein interaction specificity
NAT.STRUCT.MOL.BIOL., 11:371-379, 2004

PubMed Abstract: We developed a 'computational second-site suppressor' strategy to redesign specificity at a protein-protein interface and applied it to create new specifically interacting DNase-inhibitor protein pairs. We demonstrate that the designed switch in specificity holds in in vitro binding and functional assays. We also show that the designed interfaces are specific in the natural functional context in living cells, and present the first high-resolution X-ray crystallographic analysis of a computer-redesigned functional protein-protein interface with altered specificity. The approach should be applicable to the design of interacting protein pairs with novel specificities for delineating and re-engineering protein interaction networks in living cells.

PubMed: 15034550
DOI: 10.1038/nsmb749

Experimental method

X-RAY DIFFRACTION (2.1 Å)