1UJZ

Crystal structure of the E7_C/Im7_C complex; a computationally designed interface between the colicin E7 DNase and the Im7 Immunity protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Designed Colicin E7 immunity protein	polymer	87	9955.0	1	UniProt (Q03708) Pfam (PF01320)	Escherichia coli	dC immunity protein, Im7
2	B (B)	Designed Colicin E7 DNase	polymer	128	14706.6	1	UniProt (Q47112) Pfam (PF21431)	Escherichia coli	dC DNase
3	C, D (A, B)	water	water		18.0	91	Chemie (HOH)

Sequence modifications

A: 1 - 87 (UniProt: Q03708)

PDB	External Database	Details
Tyr 35	Asp 35	engineered mutation

B: 446 - 573 (UniProt: Q47112)

PDB	External Database	Details
Gln 528	Lys 528	engineered mutation
Arg 539	Thr 539	engineered mutation
Ala 569	His 569	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	24661.7
All*	Total formula weight	24661.7

*Water molecules are not included.