1SDB

PORCINE DESB1-2 DESPENTAPEPTIDE(B26-B30) INSULIN

Summary for 1SDB

• Entry DOI: 10.2210/pdb1sdb/pdb
• Descriptor: DESB1-2 DESPENTAPEPTIDE (B26-B30) INSULIN (3 entities in total)
• Functional Keywords: hormone
• Biological source: Sus scrofa (pig); More
• Cellular location: Secreted: P01315 P01315
• Total number of polymer chains: 2
• Total formula weight: 4979.67

Authors

Liang, D.-C.,Diao, J.-S. (deposition date: 1996-01-29, release date: 1997-02-12, Last modification date: 2024-10-23)

Primary citation

Diao, J.S.,Wan, Z.L.,Chang, W.R.,Liang, D.C.
Structure of monomeric porcine DesB1-B2 despentapeptide (B26-B30) insulin at 1.65 A resolution.
Acta Crystallogr.,Sect.D, 53:507-512, 1997

PubMed Abstract: Insulin has a concentration of 10(-8)-10(-11) M in the blood which ensures that it circulates and exerts its physiological functions in vivo as a monomer. The crystal structure of monomeric porcine desB1-B2 despentapeptide (B26-B30) insulin (DesB1-2 DPI) with M(r) = 4934 Da has been determined at 1.65 A resolution using the molecular replacement method. A structural comparison between DesB1-2 DPI and 2Zn insulin reveals that the conformation of DesB1-2 DPI is more similar to molecule I than molecule II of 2Zn insulin. The remarkable conformational difference between B25-Phe in DesB1-2 DPI and B25-Phe in despentapeptide (B26-B30) insulin (DPI) indicates that the residue B25-Phe possesses great flexibility and mobility.

PubMed: 15299880
DOI: 10.1107/S0907444997004034

Experimental method

X-RAY DIFFRACTION (1.65 Å)