1QZ8

Crystal structure of SARS coronavirus NSP9

Summary for 1QZ8

• Entry DOI: 10.2210/pdb1qz8/pdb
• Descriptor: polyprotein 1ab, SULFATE ION (3 entities in total)
• Functional Keywords: sars, replication, nsp9, coronavirus, unknown function
• Biological source: SARS coronavirus
• Cellular location: Non-structural protein 3: Host membrane; Multi-pass membrane protein (Potential). Non-structural protein 4: Host membrane; Multi-pass membrane protein (Potential). Non-structural protein 6: Host membrane; Multi-pass membrane protein (Potential). Non-structural protein 7: Host cytoplasm, host perinuclear region (By similarity). Non-structural protein 8: Host cytoplasm, host perinuclear region (By similarity). Non-structural protein 9: Host cytoplasm, host perinuclear region (By similarity). Non-structural protein 10: Host cytoplasm, host perinuclear region (By similarity). Helicase: Host endoplasmic reticulum-Golgi intermediate compartment (Potential). Uridylate-specific endoribonuclease: Host cytoplasm, host perinuclear region (By similarity): P59641
• Total number of polymer chains: 2
• Total formula weight: 25214.52

Authors

Egloff, M.P.,Ferron, F.,Campanacci, V.,Longhi, S.,Rancurel, C.,Dutartre, H.,Snijder, E.J.,Gorbalenya, A.E.,Cambillau, C.,Canard, B. (deposition date: 2003-09-16, release date: 2004-02-24, Last modification date: 2024-02-14)

Primary citation

Egloff, M.P.,Ferron, F.,Campanacci, V.,Longhi, S.,Rancurel, C.,Dutartre, H.,Snijder, E.J.,Gorbalenya, A.E.,Cambillau, C.,Canard, B.
The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world.
Proc.Natl.Acad.Sci.USA, 101:3792-3796, 2004

PubMed Abstract: The recently identified etiological agent of the severe acute respiratory syndrome (SARS) belongs to Coronaviridae (CoV), a family of viruses replicating by a poorly understood mechanism. Here, we report the crystal structure at 2.7-A resolution of nsp9, a hitherto uncharacterized subunit of the SARS-CoV replicative polyproteins. We show that SARS-CoV nsp9 is a single-stranded RNA-binding protein displaying a previously unreported, oligosaccharide/oligonucleotide fold-like fold. The presence of this type of protein has not been detected in the replicative complexes of RNA viruses, and its presence may reflect the unique and complex CoV viral replication/transcription machinery.

PubMed: 15007178
DOI: 10.1073/pnas.0307877101

Experimental method

X-RAY DIFFRACTION (2.7 Å)