1QZ8
Crystal structure of SARS coronavirus NSP9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 89.115, 89.115, 136.675 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| R-factor | 0.24951 |
| Rwork | 0.248 |
| R-free | 0.27100 * |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.880 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3.000 * | 2.840 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.056 * | 0.433 |
| Total number of observations | 100668 * | |
| Number of reflections | 9345 | |
| <I/σ(I)> | 9.4 | 1.6 |
| Completeness [%] | 99.1 | 99 * |
| Redundancy | 10.8 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.2 | 293 | Campanacci, V., (2003) Acta Crystallogr.,Sect.D, 59, 1628. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ammonium sulfate | 2.0 (M) | |
| 2 | 1 | drop | phosphate-citrate | 0.1 (M) | pH4.2 |
| 3 | 1 | drop | protein | 5.8 (mg/ml) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.8-2.2 (M) | pH4.0-4.5 |
| 5 | 1 | reservoir | phosphate-citrate | 0.1 (M) |
