1LRI

BETA-CRYPTOGEIN-CHOLESTEROL COMPLEX

Summary for 1LRI

• Entry DOI: 10.2210/pdb1lri/pdb
• Related: 1BEO 1BXM
• Descriptor: Beta-elicitin cryptogein, CHLORIDE ION, CHOLESTEROL, ... (4 entities in total)
• Functional Keywords: cryptogein, cholesterol, sterol carrier protein, toxin
• Biological source: Phytophthora cryptogea
• Cellular location: Secreted: P15570
• Total number of polymer chains: 1
• Total formula weight: 10748.90

Authors

Lascombe, M.-B.,Ponchet, M.,Venard, P.,Milat, M.-L.,Blein, J.-P.,Prange, T. (deposition date: 2002-05-15, release date: 2002-05-29, Last modification date: 2024-10-30)

Primary citation

Lascombe, M.B.,Ponchet, M.,Venard, P.,Milat, M.L.,Blein, J.P.,Prange, T.
The 1.45 A resolution structure of the cryptogein-cholesterol complex: a close-up view of a sterol carrier protein (SCP) active site.
Acta Crystallogr.,Sect.D, 58:1442-1447, 2002

PubMed Abstract: Cryptogein is a small 10 kDa elicitor produced by the phytoparasitic oomycete Phytophthora cryptogea. The protein also displays a sterol carrier activity. The native protein crystallizes in space group P4(1)22, with unit-cell parameters a = b = 46.51, c = 134.9 A (diffraction limit: 2.1 A). Its complex with cholesterol crystallizes in space group C222(1), with unit-cell parameters a = 30.96, b = 94.8, c = 65.3 A and a resolution enhanced to 1.45 A. The large inner non-specific hydrophobic cavity is able to accommodate a large variety of 3-beta-hydroxy sterols. Cryptogein probably acts as a sterol shuttle helping the pathogen to grow and complete its life cycle.

PubMed: 12198300
DOI: 10.1107/S0907444902011745

Experimental method

X-RAY DIFFRACTION (1.45 Å)