1LRI
BETA-CRYPTOGEIN-CHOLESTEROL COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 2001-12-17 |
Detector | MARRESEARCH |
Wavelength(s) | 0.964 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 30.964, 94.800, 65.296 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 * - 1.450 |
R-factor | 0.127 |
Rwork | 0.127 |
R-free | 0.16800 * |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1bxm |
RMSD bond length | 0.012 |
RMSD bond angle | 0.030 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 * | 1.480 |
High resolution limit [Å] | 1.450 | 1.448 |
Rmerge | 0.390 | 0.213 * |
Total number of observations | 64233 * | |
Number of reflections | 17432 | |
<I/σ(I)> | 16.5 | 4.1 |
Completeness [%] | 99.1 | 96 * |
Redundancy | 3.9 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | sodium chloride, cholesterol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 4.5 (M) |