1L2M
Minimized Average Structure of the N-terminal, DNA-binding domain of the replication initiation protein from a geminivirus (Tomato yellow leaf curl virus-Sardinia)
Summary for 1L2M
| Entry DOI | 10.2210/pdb1l2m/pdb |
| Related | 1L5I |
| NMR Information | BMRB: 5297 |
| Descriptor | Rep protein (1 entity in total) |
| Functional Keywords | a+b fold, rbd-like fold, viral protein |
| Biological source | Tomato yellow leaf curl Sardinia virus |
| Cellular location | Host nucleus (By similarity): P27260 |
| Total number of polymer chains | 1 |
| Total formula weight | 13583.30 |
| Authors | Campos-Olivas, R.,Louis, J.M.,Clerot, D.,Gronenborn, B.,Gronenborn, A.M. (deposition date: 2002-02-22, release date: 2002-09-18, Last modification date: 2024-05-22) |
| Primary citation | Campos-Olivas, R.,Louis, J.M.,Clerot, D.,Gronenborn, B.,Gronenborn, A.M. The structure of a replication initiator unites diverse aspects of nucleic acid metabolism Proc.Natl.Acad.Sci.USA, 99:10310-10315, 2002 Cited by PubMed Abstract: Rolling circle replication is a mechanism for copying single-stranded genomes by means of double-stranded intermediates. A multifunctional replication initiator protein (Rep) is indispensable for the precise initiation and termination of this process. Despite the ubiquitous presence and fundamental importance of rolling circle replication elements, structural information on their respective replication initiators is still missing. Here we present the solution NMR structure of the catalytic domain of Rep, the initiator protein of tomato yellow leaf curl virus. It is composed of a central five-stranded anti-parallel beta-sheet, flanked by a small two-stranded beta-sheet, a beta-hairpin and two alpha-helices. Surprisingly, the structure reveals that the catalytic Rep domain is related to a large group of proteins that bind RNA or DNA. Identification of Rep as resembling the family of ribonucleoprotein/RNA-recognition motif fold proteins establishes a structure-based evolutionary link between RNA binding proteins, splicing factors, and replication initiators of prokaryotic and eukaryotic single-stranded DNA elements and mammalian DNA tumor viruses. PubMed: 12130667DOI: 10.1073/pnas.152342699 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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