1K8Q

CRYSTAL STRUCTURE OF DOG GASTRIC LIPASE IN COMPLEX WITH A PHOSPHONATE INHIBITOR

Summary for 1K8Q

• Entry DOI: 10.2210/pdb1k8q/pdb
• Related: 1HLG
• Descriptor: Triacylglycerol lipase, gastric, 2-acetamido-2-deoxy-beta-D-glucopyranose, octyl beta-D-glucopyranoside, ... (5 entities in total)
• Functional Keywords: apha beta hydrolase fold, hydrolase
• Biological source: Canis lupus familiaris (dog)
• Cellular location: Secreted: P80035
• Total number of polymer chains: 2
• Total formula weight: 88774.90

Authors

Roussel, A.,Miled, N.,Berti-Dupuis, L.,Riviere, M.,Spinelli, S.,Berna, P.,Gruber, V.,Verger, R.,Cambillau, C. (deposition date: 2001-10-25, release date: 2002-03-06, Last modification date: 2024-11-13)

Primary citation

Roussel, A.,Miled, N.,Berti-Dupuis, L.,Riviere, M.,Spinelli, S.,Berna, P.,Gruber, V.,Verger, R.,Cambillau, C.
Crystal structure of the open form of dog gastric lipase in complex with a phosphonate inhibitor.
J.Biol.Chem., 277:2266-2274, 2002

PubMed Abstract: Fat digestion in humans and some mammals such as dogs requires the successive intervention of two lipases: gastric lipase, which is stable and active despite the highly acidic stomach environment, followed by the classical pancreatic lipase secreted into the duodenum. We previously solved the structure of recombinant human gastric lipase (HGL) at 3.0-A resolution in its closed form; this was the first structure to be described within the mammalian acid lipase family. Here we report on the open structure of the recombinant dog gastric lipase (r-DGL) at 2.7-A resolution in complex with the undecyl-butyl (C11Y4) phosphonate inhibitor. HGL and r-DGL show 85.7% amino acid sequence identity, which makes it relevant to compare the forms from two different species. The open r-DGL structure confirms the previous description of the HGL catalytic triad (Ser(153), His(353), and Asp(324)) with the catalytic serine buried and an oxyanion hole (NH groups of Gln(154) and Leu(67)). In r-DGL, the binding of the C11Y4 phosphonate inhibitor induces part of the cap domain, the lid, to roll over the enzyme surface and to expose a catalytic crevice measuring approximately 20 x 20 x 7 A(3). The C11Y4 phosphonate fits into this crevice, and a molecule of beta-octyl glucoside fills up the crevice. The C11Y4 phosphonate inhibitor and the detergent molecule suggest a possible binding mode for the natural substrates, the triglyceride molecules.

PubMed: 11689574
DOI: 10.1074/jbc.M109484200

Experimental method

X-RAY DIFFRACTION (2.7 Å)