1JCM
TRPC STABILITY MUTANT CONTAINING AN ENGINEERED DISULPHIDE BRIDGE AND IN COMPLEX WITH A CDRP-RELATED SUBSTRATE
Summary for 1JCM
| Entry DOI | 10.2210/pdb1jcm/pdb |
| Descriptor | INDOLE-3-GLYCEROL-PHOSPHATE SYNTHASE, PHOSPHATE ION, 1-(O-CARBOXY-PHENYLAMINO)-1-DEOXY-D-RIBULOSE-5-PHOSPHATE, ... (4 entities in total) |
| Functional Keywords | beta-alpha-barrel, disulphide bridge, stability mutant, lyase |
| Biological source | Escherichia coli |
| Total number of polymer chains | 1 |
| Total formula weight | 29837.11 |
| Authors | Ivens, A.,Mayans, O.,Szadkowski, H.,Wilmanns, M.,Kirschner, K. (deposition date: 2001-06-10, release date: 2002-06-10, Last modification date: 2021-10-27) |
| Primary citation | Ivens, A.,Mayans, O.,Szadkowski, H.,Jurgens, C.,Wilmanns, M.,Kirschner, K. Stabilization of a (betaalpha)8-barrel protein by an engineered disulfide bridge. Eur.J.Biochem., 269:1145-1153, 2002 Cited by PubMed Abstract: The aim of this study was to increase the stability of the thermolabile (betaalpha)8-barrel enzyme indoleglycerol phosphate synthase from Escherichia coli by the introduction of disulfide bridges. For the design of such variants, we selected two out of 12 candidates, in which newly introduced cysteines potentially form optimal disulfide bonds. These variants avoid short-range connections, substitutions near catalytic residues, and crosslinks between the new and the three parental cysteines. The variant linking residues 3 and 189 fastens the N-terminus to the (betaalpha)8-barrel. The rate of thermal inactivation at 50 degrees C of this variant with a closed disulfide bridge is 65-fold slower than that of the reference dithiol form, but only 13-fold slower than that of the parental protein. The near-ultraviolet CD spectrum, the reactivity of parental buried cysteines with Ellman's reagent as well as the decreased turnover number indicate that the protein structure is rigidified. To confirm these data, we have solved the X-ray structure to 2.1-A resolution. The second variant was designed to crosslink the terminal modules betaalpha1 and betaalpha8. However, not even the dithiol form acquired the native fold, possibly because one of the targeted residues is solvent-inaccessible in the parental protein. PubMed: 11856350DOI: 10.1046/j.1432-1033.2002.02745.x PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.1 Å) |
Structure validation
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