1JCM

TRPC STABILITY MUTANT CONTAINING AN ENGINEERED DISULPHIDE BRIDGE AND IN COMPLEX WITH A CDRP-RELATED SUBSTRATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	1999-06-15
Detector	MARRESEARCH
Wavelength(s)	0.911
Spacegroup name	P 63 2 2
Unit cell lengths	81.638, 81.638, 156.745
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.241 *
Rwork	0.241
R-free	0.31900
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.041 *	0.250 *
Number of reflections	17306
<I/σ(I)>	24.1	3.5
Completeness [%]	93.7 *	78 *
Redundancy	8.8	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	8 *	Wilmanns, M., (1990) Protein Eng., 3, 173. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	potassium phosphate	0.05 (M)	pH7.5
2	1	drop	EDTA	0.005 (M)
3	1	drop	1,4-dithioerythritol	0.002 (M)
4	1	drop		0.02 (%(w/v))
5	1	drop	protein	10-15 (mg/ml)
6	1	reservoir	potassium phosphate	0.05 (M)	pH5.0
7	1	reservoir	ammonium sulfate	1.2 (M)
8	1	reservoir	EDTA	0.005 (M)
9	1	reservoir	1,4-dithioerythritol	0.002 (M)