1IOO

CRYSTAL STRUCTURE OF NICOTIANA ALATA GEMETOPHYTIC SELF-INCOMPATIBILITY ASSOCIATED SF11-RNASE

Summary for 1IOO

• Entry DOI: 10.2210/pdb1ioo/pdb
• Related: 1BK7 1BOL 1DIX
• Descriptor: SF11-RNASE, beta-D-xylopyranose-(1-2)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[beta-D-xylopyranose-(1-2)][alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: self-incompatibility ribonuclease, hydrolase
• Biological source: Nicotiana alata (Persian tobacco)
• Cellular location: Secreted, extracellular space (By similarity): Q7SID5
• Total number of polymer chains: 2
• Total formula weight: 48377.65

Authors

Ida, K.,Sato, M.,Sakiyama, F.,Norioka, S.,Yamamoto, M.,Kumasaka, T.,Yamashita, E. (deposition date: 2001-03-26, release date: 2002-05-08, Last modification date: 2023-12-27)

Primary citation

Ida, K.,Norioka, S.,Yamamoto, M.,Kumasaka, T.,Yamashita, E.,Newbigin, E.,Clarke, A.E.,Sakiyama, F.,Sato, M.
The 1.55 A resolution structure of Nicotiana alata S(F11)-RNase associated with gametophytic self-incompatibility.
J.Mol.Biol., 314:103-112, 2001

PubMed Abstract: The crystal structure of Nicotiana alata (ornamental tobacco) S(F11)-RNase, an S-allelic glycoprotein associated with gametophytic self-incompatibility, was determined by X-ray diffraction at 1.55 A resolution. The protein has a tertiary structure typical of members of the RNase T(2) family as it consists of a variant of the (alpha+beta) fold and has eight helices and seven strands. A heptasaccharide moiety is also present, and amino acid residues that serve as the catalytic acid and base can be assigned to His32 and His91, respectively. Two "hypervariable" regions, known as HVa and HVb, are the proposed sites of S-allele discrimination during the self-incompatibility reaction, and in the S(F11)-RNase these are well separated from the active site. HVa and HVb are composed of a long, positively charged loop followed by a part of an alpha-helix and short, negatively charged alpha-helix, respectively. The S(F11)-RNase structure shows both regions are readily accessible to the solvent and hence could participate in the process of self/non-self discrimination between the S-RNase and an unknown pollen S-gene product(s) upon pollination.

PubMed: 11724536
DOI: 10.1006/jmbi.2001.5127

Experimental method

X-RAY DIFFRACTION (1.55 Å)