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CRYSTAL STRUCTURE OF NICOTIANA ALATA GEMETOPHYTIC SELF-INCOMPATIBILITY ASSOCIATED SF11-RNASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-04-12 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.02 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.869, 44.731, 64.360 |
Unit cell angles | 90.00, 90.27, 90.00 |
Refinement procedure
Resolution | 65.870 - 1.550 |
R-factor | 0.185 * |
Rwork | 0.185 |
R-free | 0.21500 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 22.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.940 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.061 | 0.307 |
Total number of observations | 237209 * | |
Number of reflections | 53199 | |
<I/σ(I)> | 14.4 | |
Completeness [%] | 97.4 | 95.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 293 | Ida, K., (2001) Acta Crystallog., D57, 143. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | cacodylate | 100 (mM) | pH6.5 |
3 | 1 | reservoir | PEG6000 | 28-34 (%(w/w)) | |
4 | 1 | reservoir | 0.8 (M) |