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1IGV

BOVINE CALBINDIN D9K BINDING MN2+

Replaces:  6ICB
Summary for 1IGV
Entry DOI10.2210/pdb1igv/pdb
Related1IG5 1clb 3icb 4icb
DescriptorVITAMIN D-DEPENDENT CALCIUM-BINDING PROTEIN, INTESTINAL, MANGANESE (II) ION (3 entities in total)
Functional Keywordscalcium-binding protein, ef-hand, manganese binding, metal binding protein
Biological sourceBos taurus (cattle)
Total number of polymer chains1
Total formula weight8565.49
Authors
Andersson, E.M. (deposition date: 2001-04-18, release date: 2001-04-25, Last modification date: 2024-02-07)
Primary citationAndersson, M.,Malmendal, A.,Linse, S.,Ivarsson, I.,Forsen, S.,Svensson, L.A.
Structural basis for the negative allostery between Ca(2+)- and Mg(2+)-binding in the intracellular Ca(2+)-receptor calbindin D9k.
Protein Sci., 6:1139-1147, 1997
Cited by
PubMed Abstract: The three-dimensional structures of the magnesium- and manganese-bound forms of calbindin D9k were determined to 1.6 A and 1.9 A resolution, respectively, using X-ray crystallography. These two structures are nearly identical but deviate significantly from both the calcium bound form and the metal ion-free (apo) form. The largest structural differences are seen in the C-terminal EF-hand, and involve changes in both metal ion coordination and helix packing. The N-terminal calcium binding site is not occupied by any metal ion in the magnesium and manganese structures, and shows little structural deviation from the apo and calcium bound forms. 1H-NMR and UV spectroscopic studies at physiological ion concentrations show that the C-terminal site of the protein is significantly populated by magnesium at resting cell calcium levels, and that there is a negative allosteric interaction between magnesium and calcium binding. Calcium binding was found to occur with positive cooperativity at physiological magnesium concentration.
PubMed: 9194174
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.85 Å)
Structure validation

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