Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 33.800, 33.800, 129.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.850 |
| Rwork | 0.186 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MG-CALBINDIN D9K |
| RMSD bond length | 0.018 |
| RMSD bond angle | 21.700 * |
| Data reduction software | XDS |
| Phasing software | SIGMAA |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.200 | 2.000 |
| High resolution limit [Å] | 1.850 | 1.860 |
| Rmerge | 0.046 | |
| Total number of observations | 17782 * | |
| Number of reflections | 6166 | |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 95.0 * | 79 |
| Redundancy | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | 295 | 65 % ammonium sulphate, 600 mM MnCl2, pH 5.6, EVAPORATION, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 35 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 65 (%) | |
| 3 | 1 | drop | magnesium sulfate | 600 (mM) |
