1GX7

Best model of the electron transfer complex between cytochrome c3 and [Fe]-hydrogenase

Summary for 1GX7

• Entry DOI: 10.2210/pdb1gx7/pdb
• Related: 1A2I 1HFE 1MDV 2CTH 2CYM
• Descriptor: PERIPLASMIC [FE] HYDROGENASE LARGE SUBUNIT, PERIPLASMIC [FE] HYDROGENASE SMALL SUBUNIT, CYTOCHROME C3, ... (9 entities in total)
• Functional Keywords: oxidoreductase, electron transfer complex, hydrogenase, multiheme cytochrome, soft docking, oxidoreductase electron transport, 4fe-4s, iron-sulfur
• Biological source: DESULFOVIBRIO VULGARIS; More
• Total number of polymer chains: 3
• Total formula weight: 65866.20

Authors

Elantak, L.,Morelli, X.,Bornet, O.,Hatchikian, C.,Czjzek, M.,Dolla, A.,Guerlesquin, F. (deposition date: 2002-03-28, release date: 2003-07-31, Last modification date: 2024-11-06)

Primary citation

Elantak, L.,Morelli, X.,Bornet, O.,Hatchikian, C.,Czjzek, M.,Dolla, A.,Guerlesquin, F.
The Cytochrome C(3)-[Fe]-Hydrogenase Electron-Transfer Complex: Structural Model by NMR Restrained Docking
FEBS Lett., 548:1-, 2003

PubMed Abstract: Cytochrome c(3) (M(r) 13000) is a low redox potential cytochrome specific of the anaerobic metabolism in sulfate-reducing bacteria. This tetrahemic cytochrome is an intermediate between the [Fe]-hydrogenase and the cytochrome Hmc in Desulfovibrio vulgaris Hildenborough strain. The present work describes the structural model of the cytochrome c(3)-[Fe]-hydrogenase complex obtained by nuclear magnetic resonance restrained docking. This model connects the distal cluster of the [Fe]-hydrogenase to heme 4 of the cytochrome, the same heme found in the interaction with cytochrome Hmc. This result gives evidence that cytochrome c(3) is an electron shuttle between the periplasmic hydrogenase and the Hmc membrane-bound complex.

PubMed: 12885397
DOI: 10.1016/S0014-5793(03)00718-X

Experimental method

SOLUTION NMR
THEORETICAL MODEL