1E97

Crystal structure of ketosteroid isomerase from Pseudomonas putida ; triple mutant y16f/y32f/y57f

Summary for 1E97

• Entry DOI: 10.2210/pdb1e97/pdb
• Related: 1C7H 1DMM 1DMN 1DMQ 1E3N 1E3R 1E3V 1OPY 4TSU
• Descriptor: STEROID DELTA-ISOMERASE (2 entities in total)
• Functional Keywords: isomerase, ketosteroid isomerase, lbhb
• Biological source: PSEUDOMONAS PUTIDA
• Total number of polymer chains: 1
• Total formula weight: 14500.50

Authors

Ha, N.-C.,Oh, B.-H. (deposition date: 2000-10-10, release date: 2001-10-05, Last modification date: 2011-07-13)

• Primary citation: Choi, G.,Ha, N.-C.,Kim, M.-S.,Hong, B.-H.,Kim, D.-H.,Oh, B.-H.,Choi, K.Y.; Peudoreversion of the Catalytic Activity of Y14F by the Additional Substitution(S) of Tyrosine with Phenylalanine in the Hydrogen Bond Network of Delta 5-3-Ketosteroid Isomerase from Pseudomonas Putida Biotype B; Biochemistry, 40:6828-, 2001; PubMed: 11389596; DOI: 10.1021/BI002767+

Experimental method

X-RAY DIFFRACTION (2 Å)