1E97
Crystal structure of ketosteroid isomerase from Pseudomonas putida ; triple mutant y16f/y32f/y57f
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 298 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 36.330, 96.530, 74.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.26400 |
| Structure solution method | OTHER |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.572 |
| Refinement software | X-PLOR (3.81) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.081 |
| Number of reflections | 9195 |
| <I/σ(I)> | 13 |
| Completeness [%] | 93.1 |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 22 * | Kim, S.W., (1997) Biochemistry, 36, 14030. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 40 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | beta-mercaptoethanol | 15 (mM) | |
| 5 | 1 | drop | d-equilenin in dimethyl sulfoxide | 10 (mM) | |
| 6 | 1 | reservoir | sodium acetate | 1.4 (M) | |
| 7 | 1 | reservoir | ammonium acetate | 0.1 (M) |
