1BDX

E. COLI DNA HELICASE RUVA WITH BOUND DNA HOLLIDAY JUNCTION, ALPHA CARBONS AND PHOSPHATE ATOMS ONLY

Summary for 1BDX

• Entry DOI: 10.2210/pdb1bdx/pdb
• Descriptor: DNA (5'-D(P*GP*CP*AP*TP*GP*CP*AP*TP*AP*TP*GP*CP*AP*TP*GP*C)-3'), HOLLIDAY JUNCTION DNA HELICASE RUVA (2 entities in total)
• Functional Keywords: dna-binding, branch migration, holliday junction, ruv, complex dna-binding protein-dna, transferase-dna complex, transferase/dna
• Biological source: Escherichia coli BL21(DE3)
• Cellular location: Cytoplasm : P0A809
• Total number of polymer chains: 8
• Total formula weight: 108039.44

Authors

Hargreaves, D.,Rice, D.W.,Sedelnikova, S.E.,Artymiuk, P.J.,Lloyd, R.G.,Rafferty, J.B. (deposition date: 1998-05-11, release date: 1999-11-24, Last modification date: 2023-08-09)

Primary citation

Hargreaves, D.,Rice, D.W.,Sedelnikova, S.E.,Artymiuk, P.J.,Lloyd, R.G.,Rafferty, J.B.
Crystal structure of E.coli RuvA with bound DNA Holliday junction at 6 A resolution.
Nat.Struct.Biol., 5:441-446, 1998

PubMed Abstract: Here we present the crystal structure of the Escherichia coli protein RuvA bound to a key DNA intermediate in recombination, the Holliday junction. The structure, solved by isomorphous replacement and density modification at 6 A resolution, reveals the molecular architecture at the heart of the branch migration and resolution reactions required to process Holliday intermediates into recombinant DNA molecules. It also reveals directly for the first time the structure of the Holliday junction. A single RuvA tetramer is bound to one face of a junction whose four DNA duplex arms are arranged in an open and essentially four-fold symmetric conformation. Protein-DNA contacts are mediated by two copies of a helix-hairpin-helix motif per RuvA subunit that contact the phosphate backbone in a very similar manner. The open structure of the junction stabilized by RuvA binding exposes a DNA surface that could be bound by the RuvC endonuclease to promote resolution.

PubMed: 9628481
DOI: 10.1038/nsb0698-441

Experimental method

X-RAY DIFFRACTION (6 Å)