1BDX
E. COLI DNA HELICASE RUVA WITH BOUND DNA HOLLIDAY JUNCTION, ALPHA CARBONS AND PHOSPHATE ATOMS ONLY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.000, 148.000, 105.600 |
| Unit cell angles | 90.00, 123.00, 90.00 |
Refinement procedure
| Resolution | ? - 6.000 |
| Structure solution method | MOLECULAR REPLACEMENT, MIR |
| Starting model (for MR) | 1cuk |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | MLPHARE |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.600 | 6.010 |
| High resolution limit [Å] | 5.700 | 5.700 |
| Rmerge | 0.043 | 0.224 |
| Total number of observations | 10906 * | |
| Number of reflections | 5263 | |
| <I/σ(I)> | 6.8 | 3.3 |
| Completeness [%] | 94.0 | 94.2 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | Hargreaves, D., (1999) Acta. Crystallogr., D55, 263. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM ACETATE | ||
| 2 | 1 | 1 | IMIDAZOLE | ||
| 3 | 1 | 2 | SODIUM ACETATE | ||
| 4 | 1 | 2 | IMIDAZOLE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | reservoir | imidazole-HCl | 0.1 (M) | |
| 3 | 1 | reservoir | sodium acetate | 0.70-0.95 (M) |
