183D
X-RAY STRUCTURE OF A DNA DECAMER CONTAINING 7, 8-DIHYDRO-8-OXOGUANINE
Summary for 183D
| Entry DOI | 10.2210/pdb183d/pdb |
| Descriptor | DNA (5'-D(*CP*CP*AP*(8OG)P*CP*GP*CP*TP*GP*G)-3') (2 entities in total) |
| Functional Keywords | b-dna, double helix, modified, dna |
| Total number of polymer chains | 1 |
| Total formula weight | 3061.99 |
| Authors | Lipscomb, L.A.,Peek, M.E.,Morningstar, M.L.,Verghis, S.M.,Miller, E.M.,Rich, A.,Essigmann, J.M.,Williams, L.D. (deposition date: 1994-08-01, release date: 1995-02-27, Last modification date: 2024-02-07) |
| Primary citation | Lipscomb, L.A.,Peek, M.E.,Morningstar, M.L.,Verghis, S.M.,Miller, E.M.,Rich, A.,Essigmann, J.M.,Williams, L.D. X-ray structure of a DNA decamer containing 7,8-dihydro-8-oxoguanine. Proc.Natl.Acad.Sci.USA, 92:719-723, 1995 Cited by PubMed Abstract: We have determined the x-ray structure of a DNA fragment containing 7,8-dihydro-8-oxoguanine (G(O)). The structure of the duplex form of d(CCAGOCGCTGG) has been determined to 1.6-A resolution. The results demonstrate that GO forms Watson-Crick base pairs with the opposite C and that G(O) is in the anti conformation. Structural perturbations induced by C.G(O)anti base pairs are subtle. The structure allows us to identify probable elements by which the DNA repair protein MutM recognizes its substrates. Hydrogen bond donors/acceptors within the major groove are the most likely element. In that groove, the pattern of hydrogen-bond donors/acceptors of C.G(O)anti is unique. Additional structural analysis indicates that conversion of G to G(O) would not significantly influence the glycosidic torsion preference of the nucleoside. There is no steric interaction of the 8-oxygen of G(O) with the phospho-deoxyribose backbone. PubMed: 7846041DOI: 10.1073/pnas.92.3.719 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.6 Å) |
Structure validation
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