12JV

Structural determination of lipid-bound Factor VIII

This is a non-PDB format compatible entry.

Summary for 12JV

• Entry DOI: 10.2210/pdb12jv/pdb
• EMDB information: 76497
• Descriptor: Coagulation factor VIII, 2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: coagulation, intrinsic pathway, hemophilia, factor viii, blood clotting
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 265891.86

Authors

Mohammed, B.M. (deposition date: 2026-04-08, release date: 2026-06-10)

Primary citation

Mohammed, B.M.
Structural determination of lipid-bound Factor VIII.
J.Thromb.Haemost., 2026

PubMed Abstract: Coagulation factor (F)VIII is the inactive precursor of FVIIIa, an essential component of the intrinsic tenase complex. Despite numerous structures of recombinant FVIII in solution, the structural basis of the procofactor-membrane interface remains poorly defined. Traditional liposome platforms require special grids and often suffer from heterogeneous particle distribution and overcrowding, precluding high-resolution single-particle analysis (SPA), while membrane mimetics, nanodiscs, are too planar posing significant biophysical challenges.

PubMed: 42202884
DOI: 10.1016/j.jtha.2026.05.021

Experimental method

ELECTRON MICROSCOPY (3.56 Å)