12JP

Structural determination of lipid-bound Factor VIII

Summary for 12JP

Entry DOI	10.2210/pdb12jp/pdb
EMDB information	76487
Descriptor	Coagulation factor VIII, 2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (4 entities in total)
Functional Keywords	coagulation, intrinsic pathway, hemophilia, factor viii, blood clotting
Biological source	Homo sapiens (human)
Total number of polymer chains	1
Total formula weight	265891.86
Authors	Mohammed, B.M. (deposition date: 2026-04-08, release date: 2026-06-10)
Primary citation	Mohammed, B.M. Structural determination of lipid-bound Factor VIII. J.Thromb.Haemost., 2026 Cited by PubMed Abstract: Coagulation factor (F)VIII is the inactive precursor of FVIIIa, an essential component of the intrinsic tenase complex. Despite numerous structures of recombinant FVIII in solution, the structural basis of the procofactor-membrane interface remains poorly defined. Traditional liposome platforms require special grids and often suffer from heterogeneous particle distribution and overcrowding, precluding high-resolution single-particle analysis (SPA), while membrane mimetics, nanodiscs, are too planar posing significant biophysical challenges. PubMed: 42202884 DOI: 10.1016/j.jtha.2026.05.021 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.46 Å)

Structure validation