11SQ
EcMscM in KCl in an open conformation, composite structure
Summary for 11SQ
| Entry DOI | 10.2210/pdb11sq/pdb |
| Related | 11SM 11SO |
| EMDB information | 76005 76007 76009 76010 76016 |
| Descriptor | Miniconductance mechanosensitive channel MscM (1 entity in total) |
| Functional Keywords | mechanosensitive, mscs, membrane protein |
| Biological source | Escherichia coli K-12 |
| Total number of polymer chains | 7 |
| Total formula weight | 873768.16 |
| Authors | |
| Primary citation | Hiotis, G.,Walz, T. The bacterial mechanosensitive channel MscM gates through concerted changes in its transmembrane and cytoplasmic domains. Nat Commun, 2026 Cited by PubMed Abstract: The mechanosensitive channel of small conductance (MscS) is the founding member of the family of MscS-like channels, which share a structurally conserved core but feature additional structural elements that define their specific channel characteristics. Here, we characterize the structure and function of the Escherichia coli mechanosensitive channel of mini conductance (MscM), which features eight additional transmembrane (TM) helices and a large periplasmic domain. Our cryo-EM structures reveal that channel gating involves conformational changes in all domains of MscM. In particular, a cytoplasmic extension of TM7 couples the conformation of the TM domain to that of the cytoplasmic domain, resulting in gating of its lateral fenestrations, where ions enter the channel. Thus, different from all other MscS-like channels studied to date, channel gating in MscM is mediated by its cytoplasmic domain and not the TM domain, which senses changes in membrane tension and operates the cytoplasmic gates. PubMed: 42481478DOI: 10.1038/s41467-026-75798-9 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.2 Å) |
Structure validation
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