11PU

Crystal Structure of viral OTU domain protease from Beiji nairovirus

Summary for 11PU

• Entry DOI: 10.2210/pdb11pu/pdb
• Related: 11NQ 11NS 11NY
• Descriptor: RNA-directed RNA polymerase L (2 entities in total)
• Functional Keywords: bjnv, l protein, norwavirus, virulance factor, hydrolase
• Biological source: Norwavirus beijiense
• Total number of polymer chains: 2
• Total formula weight: 41553.05

Authors

Gonzalez, D.S.,Pegan, S.D. (deposition date: 2026-03-09, release date: 2026-08-05)

Primary citation

Gonzalez, D.S.,Jalf, A.,Moresco, V.,Garcia, J.,Jaroszewski, L.,Matta, D.,Nguyen, J.,Torres, B.,Bergeron, E.,Godzik, A.,Pegan, S.D.
Insights into the Structure and Function of the OTU Protease Virulence Factors from Emerging Human Nairoviruses.
Acs Infect Dis., 2026

PubMed Abstract: Tick-borne negative-sense single-stranded RNA nairoviruses represent a significant global public-health threat. Over the past decade, discoveries of new orthonairoviruses and norwaviruses have surged. Many of these have been observed to cause febrile illness in humans or have been found in vectors that carry other human diseases. All orthonairoviruses encode a viral homologue of the ovarian tumor protease (OTU) superfamily, which is part of the l-protein, and the OTU is conserved in the genus. OTUs exhibit varying levels of deubiquitinating (DUB) and deISGylating activities that facilitate viral immune evasion, establishing them as key virulence factors. Here, these activities from emerging Songling, Yezo, Tacheng tick virus 1, and Pacific Coast Tick orthonairoviruses, as well as the Beiji norwavirus, were investigated. This analysis reveals a diverse range of DUB and human deISGylase enzymatic activities in the previously uncharacterized viral groups. This includes unexpected OTU human deISGylating activity and suggests that OTUs possess functions beyond their established DUB and deISGylating roles. Additionally, the molecular basis of these activities, or the absence thereof, is explored through X-ray crystallographic structures of viral OTUs encoded by four orthonairoviruses and one norwavirus. The biochemical and structural insights provide a path forward for predicting OTU activity among current and emerging nairoviruses.

PubMed: 42439547
DOI: 10.1021/acsinfecdis.6c00320

Experimental method

X-RAY DIFFRACTION (1.5 Å)