11PU
Crystal Structure of viral OTU domain protease from Beiji nairovirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.250, 73.629, 103.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.680 - 1.500 |
| R-factor | 0.1975 |
| Rwork | 0.196 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.390 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.0_5936) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.070 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.752 | |
| Rpim | 0.279 | |
| Number of reflections | 56767 | 16205 |
| <I/σ(I)> | 24.5 | |
| Completeness [%] | 98.5 | |
| Redundancy | 7.3 | |
| CC(1/2) | 0.973 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 273 | 0.1 M MES at pH 5.9, 1.2 M Sodium malonate, 30% w/v D-Sorbitol |
