11AA
Yeast co-transcriptional pre-60S assembly intermediate Nop12 RNP
Summary for 11AA
| Entry DOI | 10.2210/pdb11aa/pdb |
| EMDB information | 75580 |
| Descriptor | 25S ribosomal RNA, Ribosome biogenesis protein BRX1, 60S ribosomal protein L36-A, ... (18 entities in total) |
| Functional Keywords | ribosome biogenesis, co-transcriptional assembly, rna-binding protein, nucleolar rnp, ribosome |
| Biological source | Saccharomyces cerevisiae S288C More |
| Total number of polymer chains | 17 |
| Total formula weight | 1795108.14 |
| Authors | |
| Primary citation | Piwowarczyk, R.,Klinge, S. Mechanism for the initiation of co-transcriptional pre-60 S assembly. Biorxiv, 2026 Cited by PubMed Abstract: Eukaryotic ribosomal large subunit (60 ) assembly requires an internal transcribed spacer 2 (ITS2) to license both nucleolar and nuclear pre-60 assembly intermediates. The underlying molecular mechanisms responsible for nucleation of pre-60 assembly, quality control, and installation of ITS2 during co-transcriptional stages remain unknown. Here we report the earliest co-transcriptional assembly intermediates of the eukaryotic 60 subunits. Together with biochemical assays, our data reveal the architecture of co-transcriptional pre-60 assembly initiation and progression, as well as the molecular logic of an assembly checkpoint. This study highlights an evolutionary solution by which complex RNA folding processes can be parallelized and integrated via biological AND-gating. PubMed: 42239465DOI: 10.64898/2026.05.22.727207 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.78 Å) |
Structure validation
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