117E

THE R78K AND D117E ACTIVE SITE VARIANTS OF SACCHAROMYCES CEREVISIAE SOLUBLE INORGANIC PYROPHOSPHATASE: STRUCTURAL STUDIES AND MECHANISTIC IMPLICATIONS

Summary for 117E

DescriptorPROTEIN (INORGANIC PYROPHOSPHATASE), MANGANESE (II) ION, PHOSPHATE ION, ... (4 entities in total)
Functional Keywordsenzyme mechanism, iorganic pyrophosphatase, mutan structures, 2-metal ion mechanism, hydrolase
Biological sourceSaccharomyces cerevisiae (baker's yeast)
Cellular locationCytoplasm P00817
Total number of polymer chains2
Total molecular weight65203.21
Authors
Tuominen, V.,Heikinheimo, P.,Kajander, T.,Torkkel, T.,Hyytia, T.,Kapyla, J.,Lahti, R.,Cooperman, B.S.,Goldman, A. (deposition date: 1998-09-15, release date: 1998-12-23, Last modification date: 2011-07-13)
Primary citation
Tuominen, V.,Heikinheimo, P.,Kajander, T.,Torkkel, T.,Hyytia, T.,Kapyla, J.,Lahti, R.,Cooperman, B.S.,Goldman, A.
The R78K and D117E active-site variants of Saccharomyces cerevisiae soluble inorganic pyrophosphatase: structural studies and mechanistic implications.
J.Mol.Biol., 284:1565-1580, 1998
PubMed: 9878371 (PDB entries with the same primary citation)
DOI: 10.1006/jmbi.1998.2266
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.15 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.236802.9%0MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution