10ZZ

Yeast co-transcriptional pre-60S assembly intermediate Pwp1 RNP*

Summary for 10ZZ

• Entry DOI: 10.2210/pdb10zz/pdb
• EMDB information: 75578
• Descriptor: 25S ribosomal RNA, 60S ribosomal protein L8-A, 60S ribosomal protein L15-A, ... (8 entities in total)
• Functional Keywords: ribosome biogenesis, co-transcriptional assembly, rna-binding protein, nucleolar rnp, ribosome
• Biological source: Saccharomyces cerevisiae S288C; More
• Total number of polymer chains: 7
• Total formula weight: 1308652.09

Authors

Piwowarczyk, R.,Klinge, S. (deposition date: 2026-02-13, release date: 2026-07-01)

Primary citation

Piwowarczyk, R.,Klinge, S.
Mechanism for the initiation of co-transcriptional pre-60 S assembly.
Biorxiv, 2026

PubMed Abstract: Eukaryotic ribosomal large subunit (60 ) assembly requires an internal transcribed spacer 2 (ITS2) to license both nucleolar and nuclear pre-60 assembly intermediates. The underlying molecular mechanisms responsible for nucleation of pre-60 assembly, quality control, and installation of ITS2 during co-transcriptional stages remain unknown. Here we report the earliest co-transcriptional assembly intermediates of the eukaryotic 60 subunits. Together with biochemical assays, our data reveal the architecture of co-transcriptional pre-60 assembly initiation and progression, as well as the molecular logic of an assembly checkpoint. This study highlights an evolutionary solution by which complex RNA folding processes can be parallelized and integrated via biological AND-gating.

PubMed: 42239465
DOI: 10.64898/2026.05.22.727207

Experimental method

ELECTRON MICROSCOPY (2.8 Å)