9ZS4

S. marcescens Cas10-Csm bound to target RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)	polymer	816	90390.7	1	UniProt (A0A2I5TNR6) Pfam (PF18211) Pfam (PF22335)	Serratia	Cyclic oligoadenylate synthase
2	B (K)	crRNA	polymer	42	13361.9	1		Serratia
3	C (L)	Target RNA	polymer	42	13560.1	1		Serratia
4	D, E, F (B, C, D)	CRISPR system Cms protein Csm2	polymer	133	15886.1	3	UniProt (A0A2I5TBC8) Pfam (PF03750)	Serratia	CRISPR type III A-associated protein Csm2
5	G, H, I, J (E, F, G, H)	CRISPR system Cms endoribonuclease Csm3	polymer	248	27185.9	4	UniProt (A0A2I5TNQ0) Pfam (PF03787)	Serratia	CRISPR type III A-associated RAMP protein Csm3
6	K (I)	CRISPR system Cms protein Csm4	polymer	326	35770.6	1	UniProt (A0A2I5TQV2)	Serratia
7	L (J)	CRISPR system Cms protein Csm5	polymer	559	63148.7	1	UniProt (A0A2I5TBB3) Pfam (PF03787)	Serratia	CRISPR type III A-associated protein Csm5

Sequence modifications

B, C, D: 1 - 126 (UniProt: A0A2I5TBC8)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

E, F, G, H: 1 - 248 (UniProt: A0A2I5TNQ0)

PDB	External Database	Details
Ala 34	Asp 34	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	372633.7
All*	Total formula weight	372633.7

*Water molecules are not included.