9ZRU

Cryo-EM structure of Croatia 2023 H3N2 Influenza A hemagglutinin (HA) trimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, B, C)	HA1	polymer	334	37180.0	3	UniProt (A0A8K2AP79)	Influenza A virus
2	B, D, F (a, b, c)	HA2	polymer	238	26956.0	3	UniProt (A0A5B8WI41)	Influenza A virus
3	AA, DA, FA, G, J... (X, d, f, D, G...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	9	In PDB GlyTouCan (G42666HT)
4	BA, H, R (Y, E, O)	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	3	In PDB GlyTouCan (G22573RC)
5	CA, I, S (Z, F, P)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	3	In PDB GlyTouCan (G22768VO)
6	EA, GA, IA, K, M... (e, g, i, H, J...)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	9	In PDB GlyTouCan (G15407YE)
7	HA, N, X (h, K, U)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	3	In PDB GlyTouCan (G81315DD)
8	JA, P, Z (j, M, W)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	3	In PDB GlyTouCan (G55220VL)
9	KA, LA, MA, NA, OA... (A, B, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A, B, C: 1 - 329 (UniProt: A0A8K2AP79)

PDB	External Database	Details
Asn 53	Asp 69	conflict
Ser 96	Asn 112	conflict
Asp 122	Asn 138	conflict
Lys 140	Ile 156	conflict
Asn 145	Ser 161	conflict
Ser 156	His 172	conflict
Ala 186	Asp 202	conflict
Phe 192	Ile 208	conflict
Val 223	Ile 239	conflict
Glu 276	Lys 292	conflict
Arg 330	-	expression tag
Arg 331	-	expression tag
Arg 332	-	expression tag
Arg 333	-	expression tag
Arg 334	-	expression tag

a, b, c: 1 - 184 (UniProt: A0A5B8WI41)

PDB	External Database	Details
Gly 185	-	expression tag
Ser 186	-	expression tag
Gly 187	-	expression tag
Leu 188	-	expression tag
Val 189	-	expression tag
Pro 190	-	expression tag
Arg 191	-	expression tag
Gly 192	-	expression tag
Ser 193	-	expression tag
Gly 194	-	expression tag
Leu 195	-	expression tag
Glu 196	-	expression tag
Val 197	-	expression tag
Leu 198	-	expression tag
Phe 199	-	expression tag
Gln 200	-	expression tag
Gly 201	-	expression tag
Pro 202	-	expression tag
Gly 203	-	expression tag
Ser 204	-	expression tag
Gly 205	-	expression tag
Tyr 206	-	expression tag
Ile 207	-	expression tag
Pro 208	-	expression tag
Glu 209	-	expression tag
Ala 210	-	expression tag
Pro 211	-	expression tag
Arg 212	-	expression tag
Asp 213	-	expression tag
Gly 214	-	expression tag
Gln 215	-	expression tag
Ala 216	-	expression tag
Tyr 217	-	expression tag
Val 218	-	expression tag
Arg 219	-	expression tag
Lys 220	-	expression tag
Asp 221	-	expression tag
Gly 222	-	expression tag
Glu 223	-	expression tag
Trp 224	-	expression tag
Val 225	-	expression tag
Leu 226	-	expression tag
Leu 227	-	expression tag
Ser 228	-	expression tag
Thr 229	-	expression tag
Phe 230	-	expression tag
Leu 231	-	expression tag
Gly 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag
His 237	-	expression tag
His 238	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	192407.8
Branched	Number of molecules	30
	Total formula weight	20028.4
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	213763.4

*Water molecules are not included.