9ZR7

Cryo-EM structure of NRAS(Q61K)-BRIL fusion in complex with Fab(BAG2) and Monobody(Mb24)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	GTPase NRas,Soluble cytochrome b562	polymer	304	34441.6	1	UniProt (P12825) UniProt (P0ABE7) Pfam (PF00071)	Homo sapiens (human)	Transforming protein N-Ras,Cytochrome b-562
2	B (H)	anti-BRIL Fab, BAG2, heavy chain	polymer	228	24390.2	1		Homo sapiens
3	C (L)	anti-BRIL Fab, BAG2, light chain	polymer	213	23266.9	1		Homo sapiens
4	D (A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)

Sequence modifications

A: 1 - 174 (UniProt: P12825)

PDB	External Database	Details
Met -32	-	expression tag
Lys -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
Ser -24	-	expression tag
Ser -23	-	expression tag
Gly -22	-	expression tag
Leu -21	-	expression tag
Asn -20	-	expression tag
Asp -19	-	expression tag
Ile -18	-	expression tag
Phe -17	-	expression tag
Glu -16	-	expression tag
Ala -15	-	expression tag
Gln -14	-	expression tag
Lys -13	-	expression tag
Ile -12	-	expression tag
Glu -11	-	expression tag
Trp -10	-	expression tag
His -9	-	expression tag
Glu -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 51	Cys 51	conflict
Lys 61	Gln 61	conflict
Leu 80	Cys 80	conflict
Ser 118	Cys 118	conflict
Ala 169	Lys 169	conflict
Asp 170	Lys 170	conflict
Glu 172	Asn 172	conflict
Asp 173	Ser 173	conflict

A: 175 - 271 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 175	Met 29	conflict
Ile 270	His 124	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	82098.7
Non-Polymers*	Number of molecules	1
	Total formula weight	443.2
All*	Total formula weight	82541.9

*Water molecules are not included.