9ZPD

Cryo-EM structure of human PI3KC3-C2 in complex with Rubicon Middle Region of C terminus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Phosphoinositide 3-kinase regulatory subunit 4	polymer	1445	162838.1	1	UniProt (A0JP11)	Homo sapiens (human)
2	B (B)	Phosphatidylinositol 3-kinase catalytic subunit type 3	polymer	887	101680.3	1	UniProt (Q8NEB9)	Homo sapiens (human)	PI3-kinase type 3,PI3K type 3,PtdIns-3-kinase type 3,Phosphatidylinositol 3-kinase p100 subunit,Phosphoinositide-3-kinase class 3,hVps34
3	C (C)	UV radiation resistance-associated gene protein	polymer	699	78258.8	1	UniProt (Q9P2Y5)	Homo sapiens (human)	p63
4	D, E (D, E)	Beclin-1,Run domain Beclin-1-interacting and cysteine-rich domain-containing protein chimera	polymer	663	75300.8	2	UniProt (Q14457) UniProt (Q92622)	Homo sapiens (human)	Coiled-coil myosin-like BCL2-interacting protein,Protein GT197,Rubicon,Beclin-1 associated RUN domain containing protein,Baron
5	F (A)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
6	G (D)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 1 - 1358 (UniProt: A0JP11)

PDB	External Database	Details
Met -35	-	expression tag
Phe -34	-	expression tag
Met -33	-	expression tag
Pro -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
Phe -29	-	expression tag
Ser -28	-	expression tag
Tyr -27	-	expression tag
Ser -26	-	expression tag
Ser -25	-	expression tag
Trp -24	-	expression tag
Ala -23	-	expression tag
Thr -22	-	expression tag
Cys -21	-	expression tag
Trp -20	-	expression tag
Leu -19	-	expression tag
Leu -18	-	expression tag
Cys -17	-	expression tag
Cys -16	-	expression tag
Leu -15	-	expression tag
Ile -14	-	expression tag
Ile -13	-	expression tag
Leu -12	-	expression tag
Ala -11	-	expression tag
Lys -10	-	expression tag
Asn -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Ile -6	-	expression tag
Asp -5	-	expression tag
Gly -4	-	expression tag
Ile -3	-	expression tag
Glu -2	-	expression tag
Ala -1	-	expression tag
Thr 0	-	expression tag
Gly 1359	-	expression tag
Thr 1360	-	expression tag
Glu 1361	-	expression tag
Asn 1362	-	expression tag
Leu 1363	-	expression tag
Tyr 1364	-	expression tag
Phe 1365	-	expression tag
Gln 1366	-	expression tag
Ser 1367	-	expression tag
Gly 1368	-	expression tag
Met 1369	-	expression tag
Ala 1370	-	expression tag
Ala 1371	-	expression tag
Trp 1372	-	expression tag
Ser 1373	-	expression tag
His 1374	-	expression tag
Pro 1375	-	expression tag
Gln 1376	-	expression tag
Phe 1377	-	expression tag
Glu 1378	-	expression tag
Lys 1379	-	expression tag
Gly 1380	-	expression tag
Gly 1381	-	expression tag
Gly 1382	-	expression tag
Ala 1383	-	expression tag
Arg 1384	-	expression tag
Gly 1385	-	expression tag
Gly 1386	-	expression tag
Ser 1387	-	expression tag
Gly 1388	-	expression tag
Gly 1389	-	expression tag
Gly 1390	-	expression tag
Ser 1391	-	expression tag
Trp 1392	-	expression tag
Ser 1393	-	expression tag
His 1394	-	expression tag
Pro 1395	-	expression tag
Gln 1396	-	expression tag
Phe 1397	-	expression tag
Glu 1398	-	expression tag
Lys 1399	-	expression tag
Gly 1400	-	expression tag
Phe 1401	-	expression tag
Asp 1402	-	expression tag
Tyr 1403	-	expression tag
Lys 1404	-	expression tag
Asp 1405	-	expression tag
Asp 1406	-	expression tag
Asp 1407	-	expression tag
Asp 1408	-	expression tag
Lys 1409	-	expression tag

D, E: 1 - 450 (UniProt: Q14457)

PDB	External Database	Details
Gly 451	-	linker
Gly 452	-	linker
Ser 453	-	linker
Gly 454	-	linker
Gly 455	-	linker
Ser 456	-	linker
Gly 457	-	linker
Gly 458	-	linker
Ser 459	-	linker
Gly 460	-	linker
Gly 461	-	linker
Ser 462	-	linker

D, E: 463 - 663 (UniProt: Q92622)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	493378.9
Non-Polymers*	Number of molecules	2
	Total formula weight	588.6
All*	Total formula weight	493967.5

*Water molecules are not included.