9ZP2

E. coli RNA polymerase elongation complex containing the unnatural dP:Z*TP base pair in a trigger-loop-open conformation (b).

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, G)	DNA-directed RNA polymerase subunit alpha	polymer	231	25526.0	2	UniProt (P0A7Z4)	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (I)	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2)	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (J)	DNA-directed RNA polymerase subunit beta'	polymer	1430	158105.7	1	UniProt (P0A8T7)	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (K)	DNA-directed RNA polymerase subunit omega	polymer	74	8452.6	1	UniProt (P0A800)	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (N)	Non-template strand DNA	polymer	11	3407.2	1		Escherichia coli
6	G (R)	RNA nonamer	polymer	9	2934.8	1		Escherichia coli
7	H (T)	Template strand DNA	polymer	22	6670.3	1		Escherichia coli
8	I, J (J)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
9	K (J)	[[(2~{R},3~{R},4~{R},5~{S})-5-(5-aminocarbonyl-6-azanyl-2-oxidanylidene-1~{H}-pyridin-3-yl)-4-fluoranyl-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate	non-polymer		527.2	1	Chemie (A1C49)
10	L (J)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
11	M, N, O, P, Q... (A, G, I, J, K...)	water	water		18.0	182	Chemie (HOH)

Sequence modifications

A, G: 4 - 232 (UniProt: P0A7Z4)

PDB	External Database	Details
Asp 233	-	expression tag
Leu 234	-	expression tag

J: 1 - 1407 (UniProt: P0A8T7)

PDB	External Database	Details
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Leu 1410	-	expression tag
Glu 1411	-	expression tag
Val 1412	-	expression tag
Leu 1413	-	expression tag
Phe 1414	-	expression tag
Gln 1415	-	expression tag
Gly 1416	-	expression tag
Pro 1417	-	expression tag
Ser 1418	-	expression tag
Ser 1419	-	expression tag
Gly 1420	-	expression tag
His 1421	-	expression tag
His 1422	-	expression tag
His 1423	-	expression tag
His 1424	-	expression tag
His 1425	-	expression tag
His 1426	-	expression tag
His 1427	-	expression tag
His 1428	-	expression tag
His 1429	-	expression tag
His 1430	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	381443.6
Non-Polymers*	Number of molecules	4
	Total formula weight	682.3
All*	Total formula weight	382125.9

*Water molecules are not included.